the effect of lead on erythrocyte glucose-6-phosphate dehydrogenase activity in rats

Authors

majid sirati-sabet department of clinical biochemistry and genetics, qazvin university of medical sciences, qazvin, ir iran; cellular and molecular research center, qazvin university of medical sciences, qazvin, ir iran

gholamreza asadikaram department of biochemistry, faculty of medicine, kerman university of medical sciences, kerman, ir iran; physiology research center, institute of neuropharmacology, kerman university of medical sciences, kerman, ir iran; department of biochemistry, faculty of medicine, kerman university of medical sciences, kerman, ir iran. tel: +98-9131406916, fax: +98-3413222048

ali safari-varyani department of occupational health, qazvin university of medical sciences, qazvin, ir iran

dariush ilghari department of clinical biochemistry and genetics, qazvin university of medical sciences, qazvin, ir iran; cellular and molecular research center, qazvin university of medical sciences, qazvin, ir iran

abstract

conclusions the results of this study showed that exposure to lead increased the activity of g6pd in the rat erythrocytes, perhaps resulting in an up regulation of the enzyme to detoxify lead. materials and methods in this study 14 albino rats were divided into two groups of seven animals. the treated group was exposed to 2% lead acetate in the drinking water during eight weeks. the control group was kept in the similar condition as the test group; however this group was not exposed to lead acetate. the blood lead level was measured with an atomic absorption spectrophotometer. the g6pd activity was determined by kinetic method. the hemoglobin content was determined by drabkin's method. malondialdehyde (mda) in rats' plasma was measured with the thiobarbituric acid test using hplc. background exposure to lead damages the biological systems, developing oxidative stress. glucose-6-phosphate dehydrogenase (g6pd) produces nadph in pentose phosphate pathway. this molecule protects tissues from oxidative stress. there are conflicting reports in the literatures of the effects of lead on g6pd activity. results the blood lead concentration in treated group was increased when compared to control group (p < 0.05). a significant decrease in hemoglobin level was noted in lead-treated animals (p < 0.05). the g6pd activity in erythrocytes of rats received lead acetate increased up to 81% compared to the control group (p < 0.05). the g6pd/hemoglobin ratio in control and treatment groups was 17.7 ± 3.6 u/g and 44.9 ± 4.4 u/g, respectively. a significant increase in the plasma mda level was also observed in the exposed group (p < 0.05). objectives the aim of this investigation was to evaluate the effect of lead on erythrocyte g6pd activity in rats given lead acetate in their drinking water.

Upgrade to premium to download articles

Already have an account?login

similar resources

INHIBITION OF HUMAN ERYTHROCYTE GLUCOSE 6-PHOSPHATE DEHYDROGENASE ACTIVITY BY DEHYDROEPIANDROSTERONE AND RELATED STEROIDS.

The inhibitory effects of several steroids on G6PD activity using intact erythrocytes are reported. Incubation of whole blood with dehydroepiandrosterone (DHEA) resulted in 42% and 12% inhibition in the enzyme activity in the presence and absence of oxygen, respectively. Addition of epinephrine and/or aminophylline into the incubation medium caused further enzyme inhibition suggesting a po...

full text

Inhibition of Human Erythrocyte Glucose 6-Phosphate Dehydrogenase by Cadmium, Nickel and Aluminium

full text

incidence of erythrocyte glucose-6-phosphate- dehydrogenase deficiency

in a survey carried out to establish the incidence of erythrocyte glucose 6-phosphate-dehydrogenase deficiency, 738 professional blood donors of tehran were examined. the fluorescent spot technique was used for screening and qualitative determination of g-6-pd in erythrocytes. this technique was compared with other methods of g-6-pd enzyme assay and proved to be very reliable. qualitative enzy...

full text

the effect of aqueous extract of garlic on formalin-induced pain in male rats

چکیده اثر عصاره آبی سیر بر درد ناشی از آزمون فرمالین در موش صحرایی نر به کوشش نرگس اسکندری روزبهانی زمینه و هدف: گیاه سیر از خانواده لیلیاسه و گونهallium sativum بومی آسیای میانه بوده و از دوران ،باستان تاکنون به خواص درمانی متفاوت آن مثل: کاهندگی قندخون، کلسترول خون، فشار خون، اثرات مفیدآن بر دستگاه قلبی عروقی و بیماریهای انعقادی خون، اثرات آنتی اکسیداتیو، درمان بیماریهای تنفسی وگوارشی، ا...

15 صفحه اول

Protective Effect of Quercetin on Oxidative Stress in Glucose-6-Phosphate Dehydrogenase-Deficient Erythrocytes in Vitro

Glucose-6-phosphate dehydrogenase (G6PD) deficient subjects are vulnerable to oxidative stress. Quercetin, a flavonoids, has been employed as a potent oxygen-free radical scavenger in order to assess the protective effects of quercetin against H2O2-induced oxidative damage in G6PD-deficient and normal human erythrocytes. Erythrocytes of G6PD-deficient (n = 10) and normal (n = 10) subjects were ...

full text

Protective Effect of Quercetin on Oxidative Stress in Glucose-6-Phosphate Dehydrogenase-Deficient Erythrocytes in Vitro

full text

My Resources

Save resource for easier access later

Save to my library Already added to my library

{@ msg_add @}

Journal title:

journal of biotechnology and health sciences

جلد ۱، شماره ۱، صفحات ۰-۰

Keywords

[ ' g l u c o s e ' , 6 , ' p h o s p h a t e d e h y d r o g e n a s e ' , ' m a l o n d i a l d e h y d e ' , ' l e a d ' , ' o x i d a t i v e s t r e s s ' , ' r a t s ' ]

Hosted on Doprax cloud platform doprax.com